摘要
应用气相色谱-质谱法测定及确证鱼油制品中二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。试样采用正己烷提取,以氢氧化钾/甲醇法酯交换反应甲酯化,DB-17毛细管柱气相色谱-质谱法测定及确证,归一化法定量。添加回收率为93.3%～102.1%,变异系数2.83%～3.42%。该方法简便、快速,定性和定量准确。用于实际样品的测定取得了满意的结果。
A method for determination of eicosaqpentaenoic acid and docosahexaenoic acid in fish oil and its products by gas chromatography/mass spectrometry was developed.The sample was first extracted by n-hexane,then the fatty acid was methylated with potassium hydroxide-methanol(KOH-MeOH).The obtained fatty acid methyl esters were determined and confirmed by gas chromatograph equipped with MSD on DB-17 fused silica capillary column(30m×0.25mm×0.10μm),using normal method.The recovery was from 93.3% to 102.1% CV(n=10) was from 2.83% to 3.42%.The method was simple,rapid,accruate and applicable to determine practical samples with satisfactory results.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第10期76-78,共3页
Food Science
