摘要
将牛血清白蛋白 (BSA)吸附于强阴离子交换固定相 (SAX)上用于电色谱手性分离。当SAX吸附BSA后 ,电渗淌度仅仅下降 2 6 3% ,而电渗流的方向没有改变。在该系统中电渗流的方向和阴离子的电泳方向一致 ,因而克服了一般蛋白质固定相不能分离酸性对映体的缺点。 1种中性对映体安息香和 4种阴离子性对映体色氨酸、华法令、非诺洛芬、酮基布洛芬获得了成功分离。当流动相含体积分数为 7%的乙腈时 ,死时间和D 色氨酸、L 色氨酸的迁移时间的相对标准偏差分别为 0 90 % ,0 87%和 0 96 % (n =2 1) ,说明该体系有很好的重现性。为了缩短分析时间 ,将正己酸作为顶替试剂加入流动相中 ,结果发现对映体的保留下降很快而手性选择性减小缓慢。此外 ,还考察了乙腈的体积分数对分离的影响。
A method based on the adsorption of bovine serum albumin (BSA) on the strong anion exchange packing (SAX) was applied to prepare chiral stationary phases for capillary electrochromatography It was found that the electroosmotic mobility only decreased 26 3% when the BSA was adsorbed The run to run reproducibilities of void time and the migration times of D and L tryptophan enantiomers were rather good with the relative standard deviation (RSD) values of 0 90%, 0 87% and 0 96%, respectively, when mobile phase containing 7% (volume fraction) acetonitrile The merit of this system is that the direction of electroosmotic flow is still from cathode to anode, preferable to separate acidic enantiomers Four anionic enantiomers, tryptophan, ketoprofen, fenoprofen and warfarin, as well as the neutral enantiomers of benzoin were successfully resolved In order to reduce the analysis time, n hexanoic acid was added to the mobile phase as a competitive agent The effect of acetonitrile volume fraction on the resolution of enantiomers was also investigated
出处
《色谱》
CAS
CSCD
北大核心
2001年第5期390-394,共5页
Chinese Journal of Chromatography
