摘要
用紫外分光光度法,找出介孔碳材料对蛋白质的最佳吸附条件,并测定出该介孔碳材料对溶菌酶的最大吸附量。主要从3方面研究介孔碳材料对蛋白质吸附行为的影响。分别是介孔碳材料对蛋白质吸附行为随接触时间的变化产生的影响,pH值的改变对介孔碳材料吸附蛋白质行为的影响,以及介孔碳材料的量的改变对蛋白质吸附行为随接触时间的变化产生的影响。结果表明,当溶菌酶溶液的初始浓度为210μm ol/L,pH值为11.0,接触时间为96h时介孔碳材料对蛋白质即可达到吸附饱和。该介孔碳材料在最佳条件下对溶菌酶的最大吸附量为27.32μm ol/g,表现出优越的溶菌酶吸附性能。
The optimum contidions for adsorption of mesoporous carbon materials on protein were studied by UV spectrophotometry,and the maximum adsorbance was determined.The influnence on adsorption of mesoporous carbon materials on protein was studied from three aspect,which were as follows：the change of contact time,pH values,mesoporous carbon materials dose at different contact time.The results showed that when the initial lysozyme concentration was 210μmol/L,pH value was 11.0,mesoporous carbon materials dose was 20mg and contact time was 96h,the adsorption of mesoporous carbon materials on protein can reach the maximum efficiency.The maximum adsorption of mesoporous carbon materials on protein was 27.32μmol/g.The mesoporous carbon materials showed superior adsorption capacity on lysozyme.
出处
《光谱实验室》
CAS
CSCD
2012年第2期870-874,共5页
Chinese Journal of Spectroscopy Laboratory
基金
西安文理学院中青年专业技术人员科研资助项目(KCY200810)
西安市科技计划项目(CXY1134WL20)
关键词
介孔碳材料
溶菌酶
吸附
Mesoporous Carbon Materials
Lysozyme
Adsorption
