摘要
目的 探讨溃疡性结肠炎（ UC）上皮细胞凋亡与增殖的失调在 UC上皮屏障破 坏中所起的作用。方法 选取 35例 UC患者和 15例对照者，经肠镜取活检，作组织学检查 以明确诊断和有无异型增生。分别采用 M30 Cytodeath和 Ki－ 67两种单抗，通过免疫组织 化学 SP法检测上皮细胞凋亡和增殖。结果 UC上皮细胞凋亡指数（ M30）及凋亡增殖比与 对照组相比增高，差异有非常显著意义（ P0.05）； UC的 M30阳性凋亡细胞不仅呈灶性分 布于肠腔表面，而且隐窝底部及闭锁的腺腔内也有上皮细胞提前凋亡； UC的 Ki－ 67阳性 增殖细胞可超越隐窝下 1/3的正常范围而达上 1/3甚至隐窝顶部。结论 UC上皮细胞提前凋 亡以及凋亡和增殖比的失调也许是导致上皮屏障缺失的一个重要原因。
Objective To Investigate the disorder of colonic epithelium apoptosis and proliferation as well as its role of the epithelial barrier break down in ulcerative colitis(UC). Methods Fifty biopsy specimens were obtained endoscopically from 35 patients of UC and 15 controls respectively.The specimens were used for diagnosis of UC and investigation of epithelium proliferation an d apoptosis by IHC with markers of ki－ 67 and M30 respectively. Results Apopto sis index(marked by M30) and ratio of apoptosis to proliferation increased obvio usly in UC compared with the controls(P0.05);Disorder of epithelium apoptosis an d proliferation take place in UC.Apoptosis cells were found not only at luminal surface but also at the base of crypt;however,proliferation cells may extend to superior part of the crypt. Conclusion Unlimited and premature apoptosis as wel l as disorder of apoptosis to proliferation is maybe one of the causes which lea ding to breakdown of the epithelial barrier function i n UC.
出处
《中华消化内镜杂志》
2001年第3期161-163,T003,共4页
Chinese Journal of Digestive Endoscopy
