摘要
目的:探讨中药复方清肠栓对溃疡性结肠炎的治疗作用及其机制。方法:将SD大鼠随机分为正常组、模型组、柳氮磺胺吡啶对照组及复方清肠栓治疗组。用TNBS100mg/kg灌肠建立大鼠溃疡性结肠炎模型,第3d开始,除正常组外,各组分别给予生理盐水、柳氮磺胺吡啶、清肠栓灌肠处理,7d后处死动物,分别检测大鼠肠组织中的淋巴细胞凋亡和血清IL-1β、IL-13的含量。结果:与模型组及对照组比较,治疗组淋巴细胞凋亡明显增加(P<0.05)。与模型组相比,对照组与治疗组血清IL-1β含量均显著降低(P<0.01),IL-13含量显著升高(P<0.01);而治疗组与对照组相比,血清IL-1β、IL-13含量亦均有显著性差异(P<0.01)。结论:清肠栓可能通过促进淋巴细胞凋亡,降低血清IL-1β含量,上调血清IL-13含量,从而达到缓解TNBs诱导的溃疡性结肠炎大鼠的治疗作用。
Objective： To explore the effect and mechanism of ＂Qingchang Suppository＂ on ulcerative colitis. Methods： SD rats were randomly divided into four groups： normal group, model group, SASP group and ＂Qingehang Suppository＂ group. The rat model of ulcerative colitis was established by coloclysis of 100 mg·kg^-1 TNBs. From the third day, except for the normal group, each group was treated with an enema of 0.9% NaCI, SASP, ＂Qingchang Suppository＂ respectively. The rats were sacrificed seven days later. Apoptosis of lymphocyte in colon mucosa was detected, and the contents of sIL-1β and sIL-13 were assayed. Results： Compared with the model group and SASP group, apoptosis of lymphocytes in ＂Qingehang Suppository＂ group was increased （P〈 0.05） .Gompared with model group, the levels of sIL-1β decreased in ＂Qingchang Suppository＂ groups and SASP groups （P 〈 0.01）, and the levels of sIL-13 was increased in these groups（P 〈 0.01）. Meanwhile, there is a significant difference between ＂Qingchang Suppository＂ group and SASP group in the levels of sIL-1β and sIL-13. Conclusion：＂Qingchang Suppository＂ was effective in improving ulcerative colitis in rat model possibly by promoting apoptosis of lymphocytes in lamina propria and regulating the levels of sIL-1β and slL-13.
出处
《上海中医药大学学报》
CAS
2006年第2期37-40,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海市教委基金资助项目(04CB08)
