摘要
利用酶与酸碱相结合 ,从废弃虾蟹壳中提取几丁聚糖 ,与传统方法相比 ,具有降低成本 ,减少污染等优点 ;通过SAS系统软件得到最佳工艺参数。最佳酶解条件为 :体积比 1∶16,加酶量 140 0 0IU/ g ,pH7.0 ,温度 5 5℃ ,反应时间 5h ;脱乙酰基条件为温度 110℃ ,碱浓度4 6% ,反应时间 2h ;通过测定几丁聚糖的主要质量指标脱乙酰度和粘度 。
Chitosan was preparated from shrimp and crab shells by combination of enzyme with acid and alkali method. By comparing with the traditional method, it has saved money and protected environment The best process was got by SAS That is, when papain acted on the materials, the best proportion was water:papain=16:1,pH is 7 0, under the condition of 55℃,14?000?IU/g, for 5 hours Deacetylation was achieved by 46% NaOH at 110℃ for 2 hours By determinating deacetylation degree and viscosity, the most important evaluation factors for chitosan, this technology was proved to be feasibility.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2001年第9期42-46,共5页
Food and Fermentation Industries
基金
国家人事部留学归国人员科技活动资助项目 (人 2 0 0 10 6AD)
