摘要
以美洲大蠊 (Periplanata americana)雄性成虫的中枢神经系统为试材 ,对 Na+ -K+ - ATPase作为农药筛选靶标进行了初步研究 ,表明 Na+ - K+ - ATPase活力测定的最适反应条件是 :酶源蛋白浓度 8.5μg/ m L,温度 30℃ ,p H7.4 ,反应时间 15min。同时以最佳反应系统初步研究了几种拟除虫菊酯 (南开菊酯、氯氰菊酯、溴灭菊酯、氰戊菊酯、二氯苯醚菊酯、胺菊酯 )对 Na+ - K+ - ATPase离体活性的影响 ,结果显示出不同程度的抑制作用。并对其用作农药筛选靶标的可能性进行了探讨。
A screening model for pesticides was set up with Na + K + ATPase from the central nervous system of adult Periplaneta americana. The optimum reaction condition of Na + K + ATPase was 5～10 μg/mL for the optimal protein concentration,pH7.4,temperature 30 ℃ and time 15 min.Some pyrethroids were tested for their inhibitory activity on Na + K + ATPase,the results showed that the screening model with Na + K + ATPase is sensitive,repetitive,labor saving and sample saving.
出处
《农药学学报》
CAS
CSCD
2000年第4期28-32,共5页
Chinese Journal of Pesticide Science
