摘要
以敏感品系家蝇和溴氰菊酯抗性品系家蝇（ＭｕｓｃａｄｏｍｅｓｔｉｃａＬ．）为材料，研究和比较了神经毒剂溴氰菊酯对其脑突触体蛋白磷酸化作用的影响。结果表明，浓度为１０－５ｍｏｌ／Ｌ溴氰菊酯抑制了敏感品系家蝇脑突触体蛋白磷酸化作用，而对抗性品系家蝇脑突触体蛋白磷酸化作用无明显影响。若反应体系中加入２５×１０－６ｍｏｌ／Ｌ的ｃＡＭＰ显著激活了敏感品系家蝇脑突触体蛋白磷酸化水平，但是当０６ｍｍｏｌ／ＬＣａ２＋或０６ｍｍｏｌ／ＬＣａ２＋加１０－５ｍｏｌ／Ｌ钙调蛋白时明显增强了抗性品系家蝇脑突触体蛋白磷酸化水平，甚至超过了其对敏感品系的作用水平。此外，还发现不同浓度的溴氰菊酯可抑制突触膜上的Ｎａ／ＫＡＴＰ酶和ＣａＡＴＰ酶活力，浓度越高抑制作用也越大。
Deltamethrin was found to affect ATPase activity and protein phosphorylation of housefly brain synaptosome in vitro . Both Na,K ATPase and Ca ATPase activities can be inhibited by various concentrations of deltamethrin (10 -9 ～10 -4 mol/L). However, the inhibition was higher in susceptible housefly than in deltamethrin resistant strain. In intact susceptible housefly brain synaptosome the most significant result was inhibition of endogenous protein phosphorylation by 10 -5 mol/L of deltamethrin. Such effect in deltamethrin resistant housefly was suppressed by adding the exogenous Ca 2+ or Ca 2+ and calmodolin to the reaction system.Cyclic AMP stimulated the overall phosphorylation level,particularly 48 kD protein in susceptible housefly. The 48 kD protein,isoelectric point pI 5 2,may be the brain specific B 50 protein in housefly.All these results indicated that:(1)Deltamethrin has the property to affect ATPase activity and protein phosphorylation.(2)Deltamethrin might limits calcium transport which connects with the protein phosphorylation.
出处
《昆虫学报》
CAS
CSCD
北大核心
1999年第2期113-119,共7页
Acta Entomologica Sinica
基金
国家自然科学基金
关键词
溴氰菊酯
家蝇
脑突触膜
蛋白磷酸化
ATP酶
deltamethrin, synaptosome of housefly brain, protein phosphorylation, Na/K ATPase, Ca ATPase, deltamethrin resistant and susceptible strains of Musca domestica
