摘要
目的：检测胃癌组织及癌旁非癌组织中端粒酶活性的表达探讨端粒酶活性与各临床病理因素之间的关系。方法：采用PCR－ELISA（聚合酶链反应－酶联免疫反应）方法，定量检测68例胃癌组织及40例癌旁非癌组织中端粒酶活性表达。分别按计数和计量资料统计。结果：68例胃癌组织中，60例端粒酶活性表达（88．24％），而40例癌旁非癌组织中，6例端粒酶低活性表达（15％），两组差异极显著（P＜0．01）。胃癌组织中端粒酶活性表达与淋巴结的转移呈正相关（χ2＝4．87 u＝2．01 P＜0．05）；与组织分化呈负相关（χ2＝4．34 u＝2．17 P＜0．05）；与其他临床病理因素统计学无明显相关性。结论：端粒酶的激活与胃癌的发生发展关系密切，端粒酶活性的检测有可能成为临床胃癌诊断的辅助方法，且可能具有一定的指导术式及预后评估的意义。定量检测端粒酶活性可能更有利于揭示其在恶性肿瘤中的角色。
Objective To study the expression of telomerase activity in gastric cancer and its adjacent tissues.and to explore the relationship between telomerase activity and clinicopathological factors． Methods Telomerase activity was examined quanitatively in 68 cases of gastric cancer and 40 specimens of its nontumor adjacent tissues using polymerase chain reaction PCR－based ELISA protocol． The results were analysed by measurement and enumeration data． Results Telomerase activity was positive in 60 88．24％ of 68 gastric cancer tissues.whereas weak telomerase activity was detected only in 6 15％ of 40 normal tissues P＜0．01． And high telomerase activity was more often detected in node－positive gastric cancer tissues than that in node－negative ones χ2＝4．87 u＝2．01 P＜0．05 Telomerase activity was associated with differentiation of gastric cancer χ2＝4．34 u＝2．17 P＜0．05Telomerase activity was not statistically correlated with other clinicopathological factors． Conclusion Telomerase activity is associated with the carcinogenesis and progression of gastric cancer． Detection of telomerase activity is useful and helpful for diagnosis and radical resection of gastric cancer． Quantitative detection may be a wiser tool to discover the role of telomerase in malignant tumors．
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2001年第6期450-453,共4页
Chinese Journal of Clinical Oncology
