摘要
通过二乙基亚硝胺诱导大鼠肝细胞肝癌(hepatocellular carcinoma,HCC)的发生,采用免疫荧光、Western blot和TRAP(telomeric repeat amplication protocol)方法对端粒酶逆转录酶(telomerase reverse tran-scriptase,TERT)和端粒酶在肝癌发生过程中的动态变化及亚细胞定位进行研究.结果表明对照组及造模组的炎症期和癌旁组织中端粒酶和TERT活性较低且主要分布在细胞浆中.至肝硬化和肝癌期二者表达急增并伴随TERT的核转移.端粒酶活性升高可能与TERT的核转移有关.二者的高表达呈正相关关系(P=0.006,r=0.97)并与肝癌的发生发展相一致.
To explore the expression and subcellular location of telomerase reverse transcriptase （TERT） in the development of rat hepatocellular carcinoma （HCC） induced by diethyl nitrosoamine （DENA）, TERT is detected using western blot and immunofluorescence analysis, respectively, whereas telomerase activity is assayed using TRAP （telomeric repeat amplica- tion protocol） method. The expression levels of TERT and telomerase maintain at a relative stable lower level in normal tissue, inflammation lesion, and adjuvant normal tissue than that of those in hepatocirrhosis stage, especially in HCC stage. In precancerous lesion, TERT is mainly located in cytoplasm, whereas TERT is translocated to nuclear in hepatocirrosis and cancerous lesion. Thus, up-regulated telomerase activity might rely on TERT nuclear translocation. Over-expression of TERT and telomerase, which is positively correlated with the expression of TERT, are consistent with the progression of HCC.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第2期117-119,124,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
国家科技部基金(08C26223702027)
关键词
端粒酶逆转录酶
肝细胞肝癌
端粒酶
telomerase reverse transcriptase
hepatoceUular carcinoma
telomerase
