摘要
目的 探讨番茄红素对体外培养的人食管癌细胞的影响。方法 应用MTT法和流式细胞术手段 ,观察不同剂量的番茄红素对体外培养的人胚食管上皮癌细胞汕头株36代 (SHEEC36 )的增殖抑制及诱导凋亡作用。结果 MTT实验显示 ,番茄红素体外对SHEEC36细胞增殖有明显的抑制作用。结论 番茄红素体外能抑制SHEEC36增殖。
Objective To explore the effect of Lycopene on Shantou Huma n Embryonic Esophageal Carcinoma Cell Line, No. 36 (SHEEC36) in vitro. Methods The inhibitory rate of proliferation on SHEE36 cell li ne was determined by MTT. The fractions of each cell cycle phase and apoptosis w ere analyzed by flow-cytometry. Results Lycopene showed inhibitory effect on the proliferation in SHEEC36 cell line. The cells treated with Lycopene for 72 hours showed that the percentage of S phase decreased and G 0/G 1 phase increased significantly with the increase of do sage..Sub-G 1 peak was detected by FCM, the ratio of apoptosis increased with t he increased dosage of Lycopene .There was a significant difference at the dose of 2 μmol/L.Conclusion Lycopene can inhibit the proliferation of SHEEC36 cells in vitro, and its anticancer mechanism might be the blockage of S phase in cell cycle and the induction of cell apoptosis.
出处
《肿瘤》
CAS
CSCD
北大核心
2001年第4期246-248,共3页
Tumor
基金
广东省医药卫生青年科学研究基金资助(批准号B2 0 0 0 0 94)
关键词
食管肿瘤
细胞周期
细胞凋亡
番茄红素
肿瘤细胞
Esophageal neoplasms
Apoptosis
Cell cyc le
Lycopene
Tumor cells,cultured
