摘要
通过观察不同剂量二甲基亚砜(dimethyl sulfoxide,DMSO)作用下ECA109和PC12细胞形态和生长状态的变化,确定该溶剂对上述两种肿瘤细胞的相对安全剂量。按照一定浓度梯度(V/V)将DMSO加入培养液,干预后的24 h、48 h和72 h于倒置相差显微镜下观察AO/EB染色前后细胞的形态特征;同时用四甲基偶氮唑蓝(MTT)显色法检测细胞的生存率并计算半数抑制浓度(50%inhibiting concentration,IC50)。结果:ECA109细胞和PC12细胞对DMSO的耐受性明显不同,DMSO的浓度为8 mL/L时,24 h内ECA109细胞生长正常,形态完整,活力与对照组无差别,浓度为10 mL/L时,细胞生存率可下降10%。对PC12而言,DMSO浓度不高于14 mL/L时,对细胞的形态和生长几乎没有影响;浓度提高到20 mL/L时,24 h时细胞生存率可下降27%。结论:PC12细胞对DMSO的耐受性高于ECA109;ECA109细胞24 h内的应用终浓度不应超过10 mL/L,PC12细胞DMSO的应用浓度可提高到14 mL/L;干预时间如需延长,DMSO的加入剂量应相应降低。
The morphologic changes and growth status of ECA109 and PC12 were observed after intervened by different concentrations of Dimethyl Sulfoxide and the safe concentrations were ascertained. AO/PI staining was chosen to show the morphologic changes of ECA109 and PC12 and cellular pictures were taken before and after stained when cells were cultured full of 24 h,48 h and 72 h respectively. At the same time, cell viability and IC5o were quantitated based on the assay of MTr. ECA109 and PC12 showed different tolerance to DMSO; Except for regular growth, ECA109 also could keep perfect structure and the same cell viability as the control in 24 h if the concentration of DMSO was 8 mL/L. If the concentration was up to 10 mL/L, the cell viability would be down to 90%. To PC12 cell, the concentration of 14 mL/L showed little effect to cell＇ s morphology and growth in 24 h. If the concentration was up to 20 mL/L, the cell viability would be reduced to 73 %. It is conclused that： PC12 show higher tolerance to DMSO than ECA109 and the safe concentrations of DMSO to ECAI09 should not be above 10 mL/L but the concentration of 14 mL/L was suitable to PC12 in 24 h. If needing extension of intervention time, the corresponding concentrations of DMSO should be reduced.
出处
《科学技术与工程》
北大核心
2013年第14期3968-3971,3989,共5页
Science Technology and Engineering
