摘要
目的 :研究中药复方—益心康胶囊 (H30 3)及其各组份含药血清对氧应激所致大鼠心肌线粒体H+ -ATP酶活性损伤的保护作用。方法 :利用氧应激损伤体系 (Fe2 + VitC)与大鼠心肌线粒体共同孵育 30min ,观察对线粒体H+ -ATP酶的影响。结果 :大鼠心肌线粒体氧应激后 ,可致酶活性明显降低。单一组份研究表明 ,丹参和山楂 (6 0 0mg kg制备含药血清 )对酶活性具有明显保护作用 ,低于各组份给药量的H30 3含药血清 (10 0、2 0 0mg kg)即可产生相似的保护作用。结论 :H30 3含药血清对氧应激造成的线粒体呼吸链关键酶活性的降低具有保护作用 ,其中丹参和山楂可能是发挥作用的主要成份。
Objective The protective effects of drug-containing serum of Yi-Xin-Kang capsule, a traditional Chinese medicinal prescription, and the individual drugs of the prescription, Danshen and Shanzha, on H+-ATP ase activity of rat myocardial mitochondria injured by oxygen stress were studied.Method Myocardial mitochondria were incubated with oxygen stress injury system(Fe 2+/VitC)for 30 min, and then the activity of H+-ATPase was determined. Results Oxygen stress significantly decreased the activity of H+-ATPase of myocardial mitochondria. Treatment with the drug-containing serum of H303 or the individual drugs of Danshen and Shanzha significantly protected the activities of the enzyme. The significant protective effects were observed in lower doses(100 and 200mg/kg) of H303-treated groups and large doses (600mg/kg)of Dan-Shen or Shan-Zha-treated groups. Conclusion H303-containing serum protects the activity of H+-ATPase, a key-enzyme of myocardial mitochondria respiratory chain, injured by oxygen stress, in which Dan-Shen and Shan-Zha may contribute to the effect of H303.
出处
《中国实验方剂学杂志》
CAS
2001年第4期17-20,共4页
Chinese Journal of Experimental Traditional Medical Formulae
