摘要
目的 利用毛细管电泳法鉴别天麻及其 4种常见伪品以及测定天麻中有效成分天麻素。方法 电泳分离条件为 2 4m mol/L 硼酸盐 (p H=9.4)、运行电压为 16 k V。结果 在该分离条件下 ,通过比较电泳谱图和天麻素加标实验 ,4种伪品与天麻可以很容易鉴别 ;天麻中的天麻素可以达基线分离 ,定量测定在 15 m in之内完成 ,线性相关方程为 Y=- 0 .0 48+7.79X(r=0 .999)。结论 方法简便、快速、准确 。
Object To develop a method for the distinction of genuine Gastrodia elata B1 from its four falsified plants of common occurrence and determination of its active principle, gastrodin by capillary electrophoresis Methods The electrophoretic conditions were a buffer solution containing 24 mmol/L borate (pH=9 4), at a voltage of 16 kV Results The electrophorograms obtained under the above conditions showed easily distinguishable differences Baseline separation of gastrodin was achieved and its quantitative analysis can be accomplished within 15 min The linear correlation equation was Y=-0 048+7 79X (r=0 999) Conclusion This method could serve as an easy and precise method for the quality control of G elata
出处
《中草药》
CAS
CSCD
北大核心
2001年第4期351-352,共2页
Chinese Traditional and Herbal Drugs
基金
河北省自然科学基金!资助项目 ( 2 0 0 0 77)
关键词
毛细管电泳
天麻
天麻素
鉴别
伪品
capillary electrophoresis
Gastrodia elata Bl
gastrodin
