摘要
目的 :研究 5 -脱氧杂氮胞苷对肝癌细胞株SMMC - 772 1中E -Cadherin基因表达的影响及其机理。方法 :肝癌细胞株SMMC - 772 1用 5 -脱氧杂氮胞苷处理 ,处理前后采用流式细胞仪检测E -Cadherin基因的蛋白表达 ,观察克隆形成及裸鼠致瘤性的变化。结果 :经 5 -脱氧杂氮胞苷处理后 ,肝癌细胞株SMMC - 772 1中E -Cadherin蛋白表达增加了 43.1% ,克隆小而少 ,裸鼠致瘤性降低。结论 :E -Cadherin基因蛋白表达增高可能与DNA甲基转移酶抑制剂 5 -脱氧杂氮胞苷抑制了E -Cad herin启动子区域的高甲基化有关 ,并在一定程度上恢复了其抑癌功能。
Objective:To study the induction of 5-Aza -2 '-Deoxycytidion on the E-Cadherin gene expression in hepatocellular carcinoma ce ll line SMMC-7721 and its mechanism.Methods:Expression of E-Cadherin gene protein,clonies and tumorigenicity in nude mice of SMMC-772 1 cell before and after treatment with 5-Aza-2' deoxycytidine were observed by f low cytometer and transplantation tumor.Results:Afte r treated with 5-Aza-2'-deoxycytidine ,the expression level of E-Cadherin gene p rot ein were increased dramatically and the clonies became smaller and clony numbers were decreased, the tumorigenicities were reduced.Conclusion:These results suggested that 5-Aza-2'-deoxycytidine may not only induce the increasing expression of E-Cadhe rin protein by reactive E-Cadherin gene silenced by promoter hypermethylation,b ut also inhibite the tumor cells growth.
出处
《西北国防医学杂志》
CAS
2001年第2期154-156,共3页
Medical Journal of National Defending Forces in Northwest China
