摘要
目的:用携带分泌型抗肝癌单链免疫毒素基因(sFv-TNF-α)的重组逆转录病毒感染人外周血单个核细胞(peripheralblood mononuclear cells,PBMCs),使其表达并分泌针对人肝癌细胞的sFv-TNF-α融合蛋白,观察转导的PBMCs/PST静脉注射后对荷肝癌裸鼠的抑瘤作用.方法:分离正常人PBMCs并用PHA和IL-2进行体外刺激培养,用感染性重组病毒产生细胞C22(PA317/PST)产生的病毒上清转导后,经尾静脉输入荷肝癌裸鼠体内,注射细胞数为每只1×106(0.2 mL),每5 d注射1次,共注射3次.定期观察记录肿瘤生长情况,注射3次后处死,取出瘤组织称质量、记录,进行统计学处理,并制备石蜡切片进行常规HE染色及免疫组化染色观察.结果:实验组肿瘤生长速率为(20.8±4.9)mg/d,对照组为(28.5±6.7)mg/d,经t检验,P＜0.05.结论:经重组逆转录病毒转导的PBMCs/PST在课鼠体内对人肝癌细胞系SMMC-7721移植瘤具有一定的生长抑制作用.
AIM: To investigate in vivoantitumour activity of single-chain immunotoxin (sFv-TNF-α fusion protein). METHODS: HCC-specific killer cells were generated by transducing the recombinant retroviral virus in supernatant of the virus producing cells (C_(22)) into human peripheral blood mononuclear cells (PBMCs). SMMC-7721 xenograft nude mice were given iv either 1×10~6 (0.2mL) transduced or mock-transduced PBMCs once five days for three weeks and tumour growth was detected. RESULTS: Turnour growth were (20.8±4.9)mg/d in PBMCs/PST group and (28.5±6.7)mg/d in PBMCs/pLXSN group, with a significant difference (P<0.05). CONCLUSION: Genetic modification of PBMCs by single-chain immunotoxin has antitumour activity In vivo.
出处
《世界华人消化杂志》
CAS
2003年第6期708-711,共4页
World Chinese Journal of Digestology
基金
军队"九.五"重点补充课题
No.98M098
