摘要
用高脂高糖饲料喂养新西兰兔建立一种新的糖尿病并发动脉粥样硬化动物模型。将新西兰兔分为二组 :对照组 (n =7)喂普通饲料 ;实验组 (n =10 )喂含 10 %猪油、36 %白蔗糖混合饲料。共观察 2 4周 ,每 4周取禁食过夜空腹血测血糖、胰岛素、总胆固醇、高密度脂蛋白胆固醇和甘油三酯。结果发现 ,实验组动物发生高血糖和高甘油三酯血症 ,血糖浓度为 6 .36± 0 .92mmol L ,而对照组为 1.76± 0 .2 8mmol L ;实验组血甘油三酯浓度为 1.75± 0 .5 6mmol L ,而对照组为 0 .41± 0 .0 5mmol L ,两组相比 ,差异非常显著 (前者P <0 .0 1,后者P <0 .0 0 0 1)。实验结束时发现 ,实验组兔主动脉均有典型动脉粥样硬化早期病变 ,脂纹病灶面积占主动脉条展开面积的百分比为 8.5 8%±1.35 % ,而对照组仅有 0 .0 8%± 0 .0 6 % ,两组差异非常显著 (P <0 .0 0 1)。实验组动物脂纹病变主要分布于腹主动脉 ,类似于人类的动脉粥样硬化好发部位。此实验结果提示 :不含胆固醇的高脂高糖饮食可诱发高血糖、高甘油三酯血症 ,促发动脉粥样硬化 ,这是首例报告。
Aim To observe the effects of diabetogenic (high fat high sucrose) diet on atherogenesis in New Zealand white rabbits. Methods Two groups of New Zealand white rabbits received regular rabbit chow, or high fat high sucrose diet for 24 weeks. The levels of plasma total cholesterol, HDL cholesterol, triglycerides, insulin, and glucose were investigated, the areas of fatty streak of the aortae were measured after staining with Sodan IV, and the aortic, coronary specimens were observed with light and electron microscopies. Results The plasma glucose, and triglycerides, were significantly increased by high fat high sucrose feeding (glucose: 6.36±0.92 mmol/L vs 1.76±0.28 mmol/L, treated vs control, P<0.01; triglycerides: 1.75±0.56 vs 0.41±0.05 mmol/L, treated vs control, P<0.0001). At the end of 24 weeks, the early characteristics of atherosclerosis were present in the animals' vascular specimens (lesion areas were 8.58%±1.35% vs 0.08%±0.06%,treated vs control,P<0.001). Conclusion High fat high sucrose feeding can induce hyperglycemia, hypertriglyceridemia and atherosclerosis in New Zealand White rabbits, and this could be a potential animal model for studying the mechanisms of diabetes-accelerated a1therosclerosis. This study raised a question:What is the mechanism by which high fat high sucrose feeding induced atherosclerosis?The related hypothesis was given in this article.
出处
《中国动脉硬化杂志》
CAS
CSCD
2001年第2期100-103,共4页
Chinese Journal of Arteriosclerosis
基金
教育部留学回国人员科研启动基金
日本Otsuka医药公司新药研究基金
湖南省卫生厅科研课题基金 (985 0 )资助
关键词
致糖尿病饲料
动脉粥样硬化
血脂
血糖
Diabetogenic Diet
New Zealand White Rabbits
Atherosclerosis
Plasma Lipids
Plasma Glucose
