摘要
目的以糖尿病大鼠为实验对象,研究单纯高血糖是否可诱发动脉粥样硬化,以及游离脂肪酸在糖尿病大血管动脉粥样硬化中的作用。方法用形态学方法观察糖尿病大鼠动脉粥样硬化的表现;抽血化验游离脂肪酸、血脂和血糖水平;用免疫组织化学方法研究动脉粥样硬化局部的炎症介质表达。结果实验第3个月时光镜下可见动脉血管壁局限增厚,可观察到泡沫细胞;第5个月时已形成脂纹。糖尿病各组与对照组比较,血糖、甘油三酯和游离脂肪酸水平升高(P<0.05),一氧化氮水平降低(P<0.05)。免疫组织化学显示,白细胞介素6和肿瘤坏死因子α在糖尿病大鼠血管内膜、中膜层细胞的胞浆及细胞间质均有表达。结论单纯高血糖动物模型出现了明确的动脉粥样硬化部分特征性表现,说明高血糖是动脉粥样硬化的另一重要原因;游离脂肪酸增高可能是糖尿病动脉粥样硬化的危险因素,其作用可能与动脉壁局部炎症介质表达有关。
Ahn To explore the relationship between free fatty adds and atherosclerosis disease in diabetic rats. Methods The models of diabetic rats were made by injecting streptozecin into rat＇s abdomen. Wistar rats were divided into four groups： three diabetic groups and one control group. At one month, the third month and the fifth month, the rats＇arterys were collected as experimental material to observe atherosclerosis and to exame the expression of interleukin-6 and tumor necrosis factoraby using immunohistechemistry method, at the same time, plasma free fatty acids, cholesterol, triglyeride, hight-density lipoprotein, low-density lipoprotein, plasma glucose were examined. Results Plasma glucose, free fatty acids, triglyeride, and cholesterol level in diabetic group were higher than that in the control group and the concentration of nitric oxide in diabetes were lower than that of normal rats. Conclusions The diabetic rats model which only have hyperglycemia have atherosclerofic disease. Increase of free fatty acids may be a risk factor of atherosclerosis disease in diabetic rats.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第5期563-566,共4页
Chinese Journal of Arteriosclerosis
