摘要
采用核酸酶保护分析 (RPA)方法对番茄乙烯过表达单基因突变体Epi和野生型VFN8中LeETR1mRNA的表达特征进行了研究。结果表明 ,在正常番茄中LeETR1mRNA不受内源乙烯含量的影响 ,呈组成性表达。LeETR1mRNA在Epi部分组织中的表达强度发生了改变 ,并与Epi的形态特征变化相吻合 ,提示LeETR1在叶片的形态建成。
The expressive characteristics of LeETR1 mRNA in tomato mutant Epi and its wild type VFN8 have been studied with RNase protect assay(RPA).The results showed that LeETR1 mRNA expressed constitutionally in VFN8.And its expression was not affected by the endogenous production of ethylene.However,the high expression of LeETR1 in some tissues of Epi suggested that LeETR1 gene may have an important role in the morphogenesis of leaves,apical hooks and fruit ripening in tomato.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第2期128-132,共5页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目! ( 39870 5 12 )
