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转反义LeETR1基因番茄采后生理特性的研究 被引量:42

Characters of Postharvest Physiology of Antisense LeETR1 Transgenic Tomato Fruits
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摘要 转反义LeETR1乙烯受体基因番茄的采后生理性状与普通番茄不同。转基因番茄的呼吸作用受到抑制 ,呼吸高峰只有对照的 5 2 % ,乙烯释放高峰的出现比对照果实推迟 10d。转基因番茄的叶绿素降解和番茄红素的合成受到显著抑制 ,果实不能形成正常的红色。在成熟过程中 ,转基因番茄的纤维素酶、多聚半乳糖醛酸酶活性显著低于对照 ,延缓了果肉的软化。 In this paper we studied the post harvest physiology of antisense LeETR 1 transgenic tomato fruits.Respiratory rate of transgenic fruits was significantly lower than control.Ethylene production peak of transgenic fruits appeared 10 d after control.Chlorophyll degration and lycopene biosynthesis was inhibited in transgenic fruits.After 30 days in normal room,transgenic tomatos eventually developed an orange colour but never turned red.During the course of ripening,cellulase and polygalacturonase activity of transgenic fruits were significantly lower than control and the firmness of transgenic fruits declined more slowly.These datas suggest that LeETR 1 was closely correlated with the ripening of tomato fruits.
出处 《园艺学报》 CAS CSCD 北大核心 2003年第4期404-408,共5页 Acta Horticulturae Sinica
基金 国家自然科学基金资助项目 (3 9870 5 12 )
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