摘要
目的：探讨支气管哮喘肺内嗜酸性细胞（Ｅｏｓ）增多与Ｅｏｓ凋亡异常的关系。方法：采用卵蛋白致敏的豚鼠哮喘模型和原位细胞凋亡ＤＮＡ末端标记检测方法，动态观察肺组织Ｅｏｓ浸润、支气管肺泡灌洗液（ＢＡＬＦ）细胞学和Ｅｏｓ凋亡的变化。结果：致敏豚鼠抗原激发后，血管周围Ｅｏｓ迁移和浸润主要发生在２４ｈ以内，而支气管周围Ｅｏｓ浸润和ＢＡＬＦ内Ｅｏｓ增高持续１周以上。哮喘豚鼠第２４ｈ，４８ｈ，７２ｈＥｏｓ凋亡率分别为［（２．１±１．３）％，（３．７±２．３）％，（４．６±２．７）％，显著低于正常豚鼠（１０．１±３．４）％，Ｐ＜０．０５］，至１６８ｈ才接近于正常水平。结论：哮喘肺内Ｅｏｓ凋亡受到抑制，生存时间延长，导致肺内Ｅｏｓ增多持续存在。
Objective: To investigate the relationship between pulmonary eosinophil (Eos) infiltration and apoptotic dysregulation in asthmatic guinea pigs. Methods: After the model of asthma was established with ovalbumin sensitization in guinea pigs, the dynamic changes of pulmonary Eos infiltration, cytology of braonchoalveolar lavage fluid (BALF) and apoptosis were observed with in situ DNA tailing technique. Results: The increase in Eos infiltration around the blood vessels occurred within 24 h while that around the bronchi and increase of Eos number in BALF persisted for at least 7 d after the sensitization. The rates of Eos apoptosis in asthmatic guinea pigs at the 24th, 48th and 72nd h after the sensitization were respectively (2.1±1.3)%, (3.7±2.3)% and (4.6±2.7)%, which were significantly lower than those in the normal guinea pigs (P<0.05). Conclusion: The Eos infiltration in the lungs is inhibited to result in prolonged increase of Eos, which may play an important role in the pathogenesis of asthma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第3期198-201,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
哮喘
细胞凋亡
嗜酸性细胞
支气管肺泡灌洗
asthma
apoptosis
eosinophil
bronchoalveolar lavage fluid
guinea pig
