摘要
目的 探讨Fas单抗对嗜酸细胞 (EOS)凋亡的诱导作用及凋亡EOS的清除机制。方法分离正常人外周血EOS ,加入白细胞介素 (IL) 5与不同浓度 (1～ 10 0 0ng/ml)Fas单抗培养 ,台盼蓝拒染法和末端标记法分别检测细胞存活和凋亡变化 ,观察凋亡EOS能否被巨噬细胞 (MΦ)吞噬。结果 Fas单抗对IL 5介导的EOS存活呈浓度和时间依赖性抑制作用。高浓度的IL 5 (10 6U/L)不能抑制Fas单抗的作用。Fas单抗 (10 0ng/ml)处理 2 4h后EOS即有明显的凋亡 [(35± 6 ) % ],72h后增至 (96±3) % ,前后比较差异有显著性 (P <0 0 1)。Fas单抗 (10 0ng/ml)处理 2 4、48和 72h的EOS与MΦ培养 ,吞噬凋亡EOS的阳性MΦ百分率分别为 (2 0± 5 ) %、(38± 6 ) %和 (4 5± 6 ) % ,MΦ吞噬新分离的EOS阳性百分率为 (1± 2 ) % ,与前者比较差异均有显著性 (P <0 0 1)。结论 Fas抗体能有效诱导EOS凋亡 。
Objective Expression of the Fas antigen and induction of apoptosis in eosinophils (EOS) by anti Fas antibody in vitro were investigated. Methods The purified EOS from healthy donors were cultured for 72 h in the presence of human IL 5 and with or without anti Fas monoclonal antibody (MΦAb) at various concentrations (1～ 1 000 ng/ml) The changes of eosinophil viability, percentage of apoptosis, and fragmented DNA electrophoresis were observed The eosinophils treated by Fas MΦAb for 24 h, 48 h, 72 h were cultured with monocyte derived macrophages, the positive percentage of macrophages which engulfed EOS was calculated. Results The anti Fas MoAb suppressed IL 5 mediated EOS survival in dose and time dependant manner High concentration of IL 5 (10 6U/L) could not inhibit anti Fas MoAb mediated apoptosis The percentage of apoptosis was (35±6)% after 24 h treated with Fas MoAb ( P <0 01), and increased to (96±3)% after 72 h ( P <0 01) The percentage of macrophages which engulfed eosinophils was significantly higher than control ( P <0 01). Conclusion The anti Fas MoAb can efficiently induced eosinophils apoptosis, apoptotic EOS induced by anti Fas MoAb could be engulfed by macrophages
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2001年第3期148-151,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金资助项目 !(396 70 337)
