摘要
目的 :研究神经细胞经缺氧 缺糖培养诱导的凋亡发生、胞内钙离子变化及救脑宁注射液的治疗作用。方法 :碘化丙啶染色 ,流式细胞仪定量分析细胞凋亡百分率 ;Fluo - 3负载 ,流式细胞仪检测胞内钙平均荧光值变化。结果 :缺氧 缺糖培养可诱导神经细胞凋亡和引起细胞内钙离子增高。救脑宁注射液能抑制细胞凋亡、降低细胞内游离钙离子浓度。结论 :救脑宁注射液能抑制缺氧 缺糖诱导的神经细胞凋亡及钙超载 ,有神经细胞保护作用。
AIM: To study the effect of Jiunaoning Injection on apoptosis and the changes in cytosolic calcium induced by hypoxia/hypoglycemia in SH-SY5Y human neuroblastoma cells. METHODS: The apoptosis and the changes in cytosolic calcium were induced by hypoxia and hypoglycemia .Apoptosis was measured via flow cytometry(FCM), and cytosolic calcium was determined by Fluo-3 fluorometry. RESULTS: The hypoxia/hypoglycemia induced apoptosis and increased the cytosolic calcium in SH-SY5Y cells. Jiunaoning Injection inhibited the apoptosis of the SH-SY5Y cells and decreased the concentration of cytosolic free calcium. CONCLUSION: Hypoxia/hypoglycemia induced apoptosis and increase in cytosolic free calcium in SH-SY5Y cells, which were significantly inhibited by Jiunaoning Injection.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第4期312-314,共3页
Chinese Journal of Pathophysiology
基金
国家九五攻关课题!资助 (NO :96 - 90 6 - 0 6 - 0 1)
