摘要
为了探索“救脑宁”注射液对中风病的疗效机理 ,将原代培养 8～ 12d的新生大鼠皮层神经细胞进行缺氧缺糖处理 ,观察该药对缺氧缺糖损伤神经细胞的影响。结果表明 :缺氧缺糖组细胞上清液中丙二醛 (MDA)含量、乳酸脱氢酶 (LDH)活性较对照组显著升高 ,细胞超氧化物岐化酶 (SOD)活性和细胞生存率则显著降低 ,救脑宁组MDA、LDH显著低于缺氧缺糖组 ,而SOD活性及细胞生存率则高于缺氧缺糖组 (P <0 0 1)。因此 ,抗脂质过氧化损伤、提高神经细胞对缺氧缺糖的耐受性 ,可能是其疗效机理之一。
The cortical cells of the new born rat cultured for 8～12 days by the primary culture method were treated by oxygen and glucose deprivation (OGD), and the effects of Jiunaoning Injection (JI) on the OGD injured cortical cells were observed. The results showed that the MDA level and the LDH activity in the cell supernate were significantly increased, while the SOD activity and cell survival rate were significantly decreased in the OGD group, compared with those in the control group; and the MDA level and the LDH activity were significantly decreased, while the SOD activity and cell survival rate were increased in the JI group, compared with those in the OGD group. The results suggest that the basis of the clinical effects of JI is relieving the lesions caused by lipid peroxidation and increasing the tolerance of nerve cells to OGD.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2001年第1期28-31,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
"九五"国家科技攻关资助课题 !(No .96 90 6 0 6 0 3)
关键词
救脑宁注射液
缺氧缺糖
神经细胞
乳酸脱氢酶
中风
Jiunaoning Injection
Oxygen and Glucose Deprivation
Nerve Cell
Lactic Dehydrogenase
Lipid Oxidation
