摘要
目的 :探讨消溃灵对消化性溃疡愈合的影响及其胃粘膜保护作用的机制。方法 :采用大鼠乙酸性胃溃疡模型 ,分别以消溃灵灌胃和左旋精氨酸 (L- Arg)、亚硝基左旋精氨酸 (L- NNA)腹腔内注射治疗 7d和 14d后 ,测定溃疡指数、溃疡抑制率 ,检测血清一氧化氮 (NO)及血浆内皮素 (ET- 1)含量。结果 :消溃灵组溃疡指数、血浆 ET- 1含量明显降低 ,而溃疡抑制率、血清 NO含量显著升高 ,与模型组和 L- NNA组比较差异有显著性意义 (P<0 .0 1) ,与L- Arg组比较无统计学意义 (P>0 .0 5 )。L- NNA组溃疡指数、血浆 ET- 1水平显著高于模型组 (P<0 .0 1) ,而血清NO含量则明显低于模型组 (P<0 .0 1)。结论 :消溃灵促进溃疡愈合 ,保护胃粘膜的作用可能与其诱导、促进 NO合成 ,反馈性地抑制 ET- 1释放 ,维持 NO和 ET的动态平衡密切相关。
Objective:To explore the effect of Traditional Chinese Medicinal herbs Xiaokuiling prescription(XKL) in the healing of chronic gastric ulcer in rats and its protective effect of gastric mucosa.Method:A rat model induced by injecting acetic acid into the subserosa of stomach was used.Uicer index(UI) was measured,the level of nitri oxide (NO) in serum and plasma endothelin 1(ET 1) was examined.Results:UI and plasma ET 1 of XKL dramatically decreased,but rates of peptic ulcer controlled and the level of NO in serum obviousely increased as compared with the model group and L NNA group(P< 0 01 ),while UI and plasma ET 1 of L NNA group were higher than those of model group(P< 0 01 ),the level of NO in serum was lower than that of model group.Conclusion:XKL could improve the healing of gastric ulcer and protect gastric mucosa,the possible mechanism in closely related to the increase of NO and the reduction of ET 1 so that the balance of NO and ET could be maintained.
基金
国家中医药管理局科研基金资助项目 (No.95 B15 1)
