摘要
目的 :探讨中药消溃灵方对消化性溃疡愈合的影响及其对胃粘膜的保护机制。方法 :采用大鼠乙酸性胃溃疡模型 ,用游标卡尺测定溃疡指数 ,分别用放射免疫和免疫组织化学方法观察血浆内皮素 (ET-1 )含量变化、表皮生长因子受体 (EGFR)在溃疡周围粘膜的表达及图象分析。结果 :消溃灵组和 L-Arg组溃疡指数明显低于模型组 (P<0 .0 5 ) ,极显著低于 L-NNA组 (P<0 .0 1 ) ;L-NNA组溃疡指数明显高于模型组 (P<0 .0 5 )。消溃灵组和 L-Arg组血浆 ET-1含量明显低于对照组 (P<0 .0 5 ) ,显著低于模型组 (P<0 .0 5 ) ,极显著低于 L-NNA组 (P<0 .0 1 ) ;L-NNA组血浆 ET-1含量显著高于模型组及对照组 (P<0 .0 1 )。消溃灵组和 L-Arg组 EGFR表达较模型组明显增加(积分光密度值及阳性细胞占总面积百分比均 P<0 .0 5 ) ,较 L-NNA组和对照组显著增加 (积分光密度值及阳性细胞占总面积百分比均 P<0 .0 1 ) ;L-NNA组 EGFR较模型组显著减弱 (积分光密度值及阳性细胞占总面积百分比均 P<0 .0 1 ) ;消溃灵组溃疡指数、血浆 ET-1含量及表皮生长因子受体 (EGFR)在溃疡周围粘膜的表达与 L-Arg组比较无明显差异 (P>0 .0 5 )。结论 :消溃灵能抑制 ET-1的释放 ,促进溃疡周围 EGFR的表达以保护胃粘膜 ,促进溃疡愈合 ,其作用可能与
Objective: To investigate the effect and mechanism of Xiaokuiling(XKL) on healing chronic gastric ulcer and protecting gastric mucosa in rats.Methods:The rats gastric ulcer was induced by acetic acid.The index of ulcer(UI) was measured by a vernier,and the level of plasma endothlin 1(ET 1) were determined by radiolmmunossay,and the expression of epidermal growth factor receptor (EGFR) around ulcer was detected by immunohistochemical method(SABC),and the microimagine was analysed by MPISA.Results: The UI in XKL group and L Arg group were significantly lower than those in model groupa and L NNA group( P <0.05, P <0.01);the UI in L NNA group was significantly higher than that in model group.The level of plasm ET 1 in L NNA group was obviously higher than those in model group and control group( P <0.01, P <0.01).The express of EGFR in XKL group and L Arg group was significantly increased compared with that in model group( P <0.01),and was extremely increased compared with that in control group( P <0.001).The expression of EGFR in L NNA group was obviously weakened compared with that in model group( P <0.05). Conclusion:XKL can inhibit the release of ET 1 and promote the expression of EGFR around ulcer,so as to protect the gastric mucosa and make ulcer heal up.The effection of XKL on ET 1 and EGFR may be responsible for the synthesis of NO.
出处
《浙江中西医结合杂志》
2003年第1期4-6,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
国家中医药管理局科研基金资助项目 ( N O:95B151)
