摘要
目的 探讨非特异性酯酶 (Non- specific esterase,NSE)在淡色库蚊对不同杀虫剂抗性中的作用。 方法 以 β-乙酸萘酯为底物 ,坚固蓝 B盐为显色剂 ,测定室内 5个品系淡色库蚊的 NSE活力。 结果 5个品系淡色库蚊中以抗DDVP品系 NSE活力水平最高 ,其次为抗 DDVP降解品系和抗残杀威品系。抗氯氰菊酯品系较低 ,与敏感品系相近。 结论 NSE在淡色库蚊对有机磷类杀虫剂的抗性中起重要作用 ,也是对氨基甲酸酯类杀虫剂产生抗性的机制之一 。
Objective To explore the role of non specific esterase (NSE) of Culex pipiens pallens in resistance against insecticides. Methods Laboratory detection of NSE was performed for 5 strains of the mosquito using β Naphthyl acetate as the substrate and fast blue B salt solution as the color agent. Results NSE level was highest in anti DDVP strain, followed by anti DDVP degenerated strain and anti propoxur strain, while the level of NSE of anti cypermethrin was similar to that of susceptible strain. Conclusion NSE was showed to play important role in the mosquito resistance against organophosphorus insecticides and was one of important mechanism for the development of resistance towards carbamates, while it has little relation with the resistance against pyrethroids.
出处
《中国寄生虫病防治杂志》
CSCD
2001年第1期52-53,共2页
Chinese Journal of Parasitic Disease Control
基金
山东省卫生厅
济宁市科委
济宁医学院青年科研基金资助项目
关键词
淡色库蚊
抗药性
非特异性酯酶
杀虫剂
Culex pipiens pallens
insecticide resistance
non specific esterase
