摘要
目的 :了解高效氯氰菊酯对德国小蠊乙酰胆碱酯酶和非特异性酯酶活性的影响 ,为探索抗性机制及抗药性生化检测方法提供理论依据。方法 :采用Groun改进的Ellman方法和Valles方法分别测定施用不同浓度高效氯氰菊酯后 2 4h和 3 0h德国小蠊乙酰胆碱酯酶的酶活性及施药后 2 4h后德国小蠊非特异性酯酶的活性。结果 :随着施药浓度增加 ,乙酰胆碱酯酶酶活性受抑制程度逐渐增加 ,施药浓度在 0 .0 0 2 %以上各剂量组的乙酰胆碱酯酶酶活性与对照组相比 ,差异均有显著性。各施药组和对照组非特异性酯酶活性差异无显著性。结论
Objective: To measure the effect of beta-cypermethrin to the general esterase and acetylcholine esterase activity of German cockroach.Methods:Treatments were made by topically applying different concentration beta-cypermethrin to adult male German cockroaches (Blattella germanica).Then general esterase and acetylcholine esterase activity was measured respectively with Valles' method and Ellman's method which improved by Groum. Results:The results showed that AchE activity of Blattella germanica decreased correlated with the concentration of beta-cypermethrin increasing and the AchE activity of the treated group was significantly lower than the contrast group.Conclusion: This indicated that beta-cypermethrin can inhibit the activities of acetylcholine.Acetylcholine esterase is the main target enzyme of beta-cypermethrin.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第6期416-418,共3页
Chinese Journal of Vector Biology and Control
基金
铁道部站车卫生科研基金资助项目 (编号J99Z2 1 7)
