摘要
目的 观察富含丙氨酰谷氨酰胺 (Ala Gln)和支链氨基酸 (BCAA)的氨基酸注射液 ,对创伤大鼠的营养支持效果。 方法 Wistar大鼠 40只 ,行创伤和中心静脉插管后 ,随机分为无氮组、正常饲料组、对照组和实验组。测定饲料摄入量、体重、氮平衡、血浆蛋白和氨基酸含量、外周血淋巴细胞转化率、伤口埋植海绵中羟脯氨酸含量、皮肤抗张力强度和小肠粘膜蛋白质及核酸。 结果 无氮组动物营养状况欠佳 ,各项指标均较差。实验组动物的血浆谷氨酰胺 (Gln)、BCAA含量、外周血淋巴细胞转化率、伤口皮肤抗张力强度和羟脯氨酸含量均显著高于对照组和正常饲料组 (P <0 .0 5 )实验组和正常饲料组动物第 8天氮平衡 ,小肠粘膜核酸和蛋白质明显高于对照组 (P <0 .0 5 )。 结论 Gln和BCAA有助于改善创伤后机体的营养及代谢状况 ,增强免疫功能 ,促进小肠粘膜细胞增殖。
Objective To observe the effects of amino acids solution rich with alanylglutamine(Ala-Gln)and branched-chain amino acids(BCAA)on traumatized rats. Methods After injured and centeral vena catheter placed,40Wistar male rats were divided into nitrogen-free,normal diet,control and experimental groups randomly.Dietary intake was weighed everday and body weight every other day. On days 4 and 8,nitrogen balance was measured.On days 14,serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined.At last,the animals were killed,the hydroxyproline and tensile strength of wound,and intestinal mucosal DNA,RNA and prontein content were examined. Results Nitrogen-free group in all variance was worser than other groups.The nitrogen balance on days 8 was remarkably improved in experimental and normal diet groups compared with control group.There were higher serum glutamine(Gln) and BCAA concentration,hydroxyproline content and tensile strength of wound,and better lymphocyte blastogenesis,experimental group compared with control and normal diet groups.Experimental and normal diet groups had higher mucosal DNA,RNA and protein content than control group. Conclusions Gln and BCAA can optimize hypercatabolism,enhance immunity and promote wound healing and the growth of intestinal mucosa.
出处
《中国临床营养杂志》
2001年第1期18-19,共2页
Chinese Journal of Clinical Nutrition
