摘要
利用毛细管电泳 -电化学检测技术 (CE -EC)对牛膝多糖水解液中的单糖进行了分离测定 ,证实牛膝多糖的化学组成中主要有葡萄糖、甘露糖和果糖3种单糖组分 ,并对其含量进行了测定。讨论了工作电位、分离电压、缓冲液浓度等因素对分离检测的影响。
A new method for the separation and determination of monosacchride components in the polysaccharide of Radix Achyranthis Bidentatae is described. The polysaccharide was hydrolyzed in three different ways, and three different monosaccharides separated and determined by CE-EC were proved to be glucose, mannose and fructose. The experimental conditions affecting determination such as detection potential, separation voltage and buffer concentration were studied and optimized.
出处
《分析测试学报》
CAS
CSCD
2000年第5期53-55,共3页
Journal of Instrumental Analysis
基金
中国科学院长春应用化学研究所电分析化学开放实验室资助项目
关键词
毛细管电泳
电化学检测
牛膝
多糖
单糖
中药分析
Capillary electrophoresis
Electrochemical detection
Radix Achyranthis Bidentatae
Polysa_ccharide
Monosaccharide
