摘要
目的：研究牛膝对Ｐ３８８白血病细胞的体外细胞毒性及其活性成分。方法：牛膝根以水提取，经Ｔｏｙｏｐｅａｒ１ＨＷ－４０Ｃ凝胶过滤，得到高分子物质部分。以Ｐ３８８白血病细胞的体外培养确定细胞毒活性。以ＳｅｐｈａｃｒｙｌＳ－２００进行分离和纯化。以１Ｈ－ＮＭＲ进行初步的组成鉴定。结果：牛膝水提取物的高分子物质部分有细胞毒性，ＩＣ５０值为５．２μｇ·ｍｌ－１。多糖为其主要成分，由木糖、甘露糖和果糖等单糖做成。结论：牛膝水提取物的高分子物质部分对Ｐ３８８白血病细胞有显著的体外细胞毒性。多糖可能是细胞毒性成分。
OBJECTIVE:To study the active composition of the extract from Achyranthes bidentata root.Bl.(amaranthaceae) and its cytotoxicity against P388 leukemia cells in vitro. METHODS:Water macerating extract of A.bidentata root was obtained and isolated by Toyopearl HW 40C gel filtration to get a high molecular part. In vitro cytotoxicity was based on culturing P388 leukemia cells.Active composition was seperated and purified with Sephacryl S-200 from the high molecular part and identified with 1 H NMR spectrum.RESULTS:Cytotoxicity (IC 50 5.2μg·ml -1 ) was detected and a polysaccharide was found to be a main composition of the high molecular part,which was composed of xylose,mannose,fructose and so on.CONCLUSION:High molecular part from water macerating extract of A.bidentata root has a remarkable cytotoxicity against P388 leukemia cells in vitro. The polysaccharide of A.bidentata root may be cytotoxic.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1999年第5期299-301,共3页
Chinese Pharmaceutical Journal
关键词
牛膝
细胞毒性
白血病
P388细胞
中医药疗法
Achyranthes bidentata, cytotoxicity,P388 leukemia cells,polysaccharide,high molecular extract
