摘要
目的 :了解供者淋巴细胞输注 (DLI)后 ,产生GVL效应的克隆性TCRVβ亚家族T细胞的情况。 方法 :利用RT PCR分别扩增 2例CML经allo BMT后复发病人DIL治疗前后不同时间外周血单个核细胞的TCRVβ 2 4个亚家族基因的CDR3,了解病人各Vβ亚家族的利用情况。阳性的PCR产物进一步经荧光素标记和基因扫描分析产物的CDR3长度 ,了解T细胞克隆性。结果 :DLI治疗前病人外周血仅表达 4～ 11个Vβ亚家族 ,而DLI治疗后缓解期则可检测到 12～ 2 1个Vβ亚家族。基因扫描显示DLI后病人外周血出现某些新的Vβ亚家族克隆性增殖T细胞。结论 :病人的TCRVβ亚家族T细胞存在倾斜性分布现象 ,该倾斜现象在完全缓解时逐步恢复正常。DLI治疗病人时 。
Objective:To investigate the clonal expansion of TCR Vβ subfamily T cells which were considered as GVL effective cells after donor lymphocytes infusion (DLI) in patients with relapse CML after allo BMT Methods:The CDR3 of TCR Vβ 24 subfamily genes were amplified in samples of peripheral blood mononuclear cells at different time points before and after DLI, which were drawn from 2 cases with relapse CML treated by allo BMT, to observe the usage of TCR Vβ repertoire, the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluating clonality of the detectable TCR Vβ T cells Results:Only 4 11 Vβ subfamily T cells could be identified in CML cases before DLI, and 12 21 Vβ subfamily T cells could be detected in samples from CML which display remission after DLI Genescan analysis showed that new clonal expansion TCR Vβ subfamily T cells could be found in samples after DLI Conclusion:The skew distribution of TCR Vβ subfamily T cells could be found on patients with relapse CML after allo BMT, and this skewing pattern may stage to stage to normal pattern during the complete remission The GVL effect may exert through some clonal expansion TCR Vβ subfamily T cells, during the treatment of DLI in relapse CML
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第3期157-160,共4页
Chinese Journal of Immunology
基金
国家教育部<高等学校骨干教师资助计划>基金资助项目! (教技司[2 0 0 0 ] 6 5号 )
广东省科委重点科技项目 !( 2KM0 5 40 3s)
广
关键词
供者淋巴细胞输注
慢性粒细胞白血病
T细胞受体β基因
T细胞克隆
异基因骨髓移植
Donor lymphocytes infusion Chronic myelogenous leukemia T cell receptor Vβ gene T cell clonality Allogeneic bone marrow transplantation
