摘要
目的:了解急性髓性白血病(AML)M2a细胞诱导的体外增殖T细胞的特异性杀伤作用和克隆性增殖情况。方 法:利用肿瘤细胞-T淋巴细胞混合培养方法(MLTC),分别收集3例经AML-M2a细胞体外诱导不同扩增时间的健康人T细 胞,利用LDH释放方法分析其特异性细胞毒作用,同时利用RT-PCR和基因扫描方法分析经AML-M2a细胞诱导前后T细胞 的24个TCR Vβ亚家族基因的表达和克隆性情况。结果:在培养第12天和第21天时,1例AML-M2a细胞诱导的3例健康人 T细胞对该诱导细胞的平均杀伤率分别为39.88±7.79％和62.14±9.79％,而对AML-M2a患者和健康人外周血T细胞均无 杀伤作用。TCR Vβ谱系分析发现诱导后3例健康人T细胞均出现Vβ16的克隆性增殖。结论:AML-M2a细胞可诱导健康人 T细胞成为具有克隆性增殖的特异性CTL,并以Vβ16的克隆性增殖为主。
Objective: To investigate the clonal expansion and specific cytotoxicity of TCR Vβ subfamily T cells from healthy individuals induced by acute myelogenous leukemia M2a subtype (AML-M2a) cells, respectively. Methods: T cells from three healthy individuals that were induced by AML-M2a cells by using mixed lymphocyte and tumor culture ( MLTC) in different amplified stage were detected for the specific cytotoxicity by LDH release assay. The distribution and clonal expansion of TCR Vβ 24 subfamily genes of T cells before and after induction were analyzed by RT-PCR and genes-can. Results: At 12th and 21st day after culture the cytotoxicity rates of three healthy individuals T cells after MLTC for the induced cells. (AML-M2a cells) were 39. 88±7. 79% and 62.14±9. 79% , respectively, whereas there were not any cytotoxicity in the T cells of peripheral blood from the AML-M2a case and three healthy individuals. Clonal expansion of TCR Vβ16 subfamily T cells was displayed by genescan in the T cells from three donors after MLTC. Conclusions: The clonal expansion and specific cytotoxicity T cells from healthy individuals could be induced by AML-M2a cells. The clonal expansion T cells were more tended to TCR Vβ16 subfamily.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2004年第3期195-199,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金(39870358)广东省自然科学基金(980705)广州市科委重点科技攻关计划(98-z-039-01)广东省医学科研基金(A1999271)资助
