摘要
目的评价丝裂霉素Ｃ（ＭＭＣ）对人翼状胬肉成纤维细胞（ＨＰＦ）增殖的影响。方法体外培养ＨＰＦ并传５－８代，分别加入１０５，１０４，１０３，１０２，１０和１μｇ／Ｌ不同质量浓度的ＭＭＣ，对照组加入磷酸盐缓冲液（ＰＢＳ），４８ ｈ后用噻唑兰（ＭＴＴ）比色法在酶标仪上测吸光度Ａ，计算各质量浓度下ＨＰＦ的净抑制率。结果实验组与对照组Ａ值比较有显著性差异（Ｐ＜０．０５），质量浓度在１０２μｇ／Ｌ以上尤甚（Ｐ＜０．０１）。ＭＭＣ对ＨＰＦ增殖的抑制率与其药液质量浓度呈正相关。结论ＭＭＣ具明显抗ＨＰＦ增殖作用，并与用药质量浓度呈良好的量效关系。
Objective To evaluate the inhibiting effects of mitomycin C(MMC ) on fibroblasts of human pterygium (HPF) in vitro. Methods The fibroblasts derived from the 5 th to 8 th generation of the cultured tissues in human pterygium. These fibroblasts were exposed to culture plates with MMC at 6 gradient concentrations, adjusting the concentration value of the culture medium to 105, 104, 103 , 102, 10 and 1 μg/L. The cells had been treated with PBS in the control group. After 48 h of incubation , the absorbency had been determined individually by MTT[ 3 - (4,5 - dimethylthiazol-2-yl) -2, 5 - diphenyl- tetrazolium bromide ] method. The inhibitory rates were calculated accordingly. All the data were analyzed with student t test and linear regression. Results The extensive breakage of fibroblasts was observed. There was a statistical difference between the tested group and control group in absorbency (P < 0. 05 ) , especially at the concentrations of more than 100 iμ/L (P < 0. 01 ). The degree of growth inhibition increased with exposure of cells to MMC over 48 h. It showed that there is a positive correlation between the doses of MMC and the antiproliferative effects on HPF. Conclusion MMC significantly inhibites the proliferation of HPF in a dose- dependent manner.
出处
《眼科研究》
CSCD
北大核心
2001年第1期39-41,共3页
Chinese Ophthalmic Research
