摘要
通过光学显微镜、荧光显徽镜、电子显微镜、琼腊糖凝胶电泳、流式细胞术等方法,探讨不同质量浓度丝裂霉素作用不同时间后,K562细胞凋亡的情况.结果表明,丝裂霉素能够诱导K562细胞凋亡,作用48 h的最佳质量浓度为12.5μg/mL;最适质量浓度诱导细胞凋亡的凋亡率为(28.8±1.04)%(P<0.01);丝裂霉素可影响K562细胞增殖周期中的S期,即DNA合成期,细胞在此期停滞,诱导其发生凋亡.用丝裂霉素可起到诱导K562细胞凋亡的作用,这对于指导临床用药,具有重要意义.
The multiplication and apoptosis status of K562 cells treated different time by different concentration mitomycin were observed by light microscope,fluorescence microscope,TEM,agrose gel electrophoresis of DNA and flow cytometry.The results showed that MMC could induce K562 cells apoptosis,and the best concentration was 12.5μg/mL for 48 h.The optimal concentration of apoptosis induced by apoptosis rate is（28.8±1.04）%（P0.01）.Mitomycin could affect the S phase among cellular multiplication,cell could be blocked by mitomycin and then apoptosis in this phase.Mitomycin can induce the apoptosis of human leukemic K562 cells.It is of great significance to guide clinical medication.
出处
《高师理科学刊》
2012年第3期62-66,共5页
Journal of Science of Teachers＇College and University
基金
黑龙江省自然科学基金项目(C200624)
黑龙江省教育厅科学技术研究项目(11511447
12511611)
