摘要
目的 :对内毒素 (LPS)直接作用于血管内皮细胞 (VECs)激活的多种信号通路的主要激酶及转录因子进行筛选研究。方法 :10 0ng·ml-1浓度的LPS刺激细胞至预定时相点后 ,用免疫细胞化学观察信号分子的磷酸化和 /或核转位。结果 :LPS直接作用于VECs在 5～ 15min内即可观察到多种信号分子的激活 ,随刺激时间延长 ,信号分子激活程度逐步增强 ,至一定时相点后活性又消失 ,这些信号分子的活性在持续时间上存在一定的差异。这些激活的信号分子包括p38、ERK1/ 2MAPK、CREB、STAT5、STAT6、NF -kB家庭成员p6 5、p5 0、p5 2、c-Rel、Rel-B等。结论 :LPS直接作用于VECs可以迅速激活多种信号分子 ,诱导信号分子的磷酸化和 /或核转位。LPS诱导的CREB磷酸化、不同NF -kB家族成员核转位持续时间的差异是第一次报道。
Objective:This study is aimed at the screning of kinases and transcription factors,which are activated when vascular endothelial cells(VECs),are stimulated by LPS directly.Methods:Immunocytochemistry was used to observe the phosphorylation and /or nuclear translocation of signal molecules when VECs were stimulated by 100 ng·ml 1 LPS under different time intervals.Results:Activation of several signal molecules was observed when stimulated as early as 5 to 15 minutes and enhanced following the elongation of stimulation time.The lasting times of activation were different.These activated signal molecules included p38,ERK1/2MAPK,CREB,STAT5,STAT6and members of NF-kB family,which were p65,p50,p52,c-Rel and Rel-B.Conclusion:Phosphorylation and/or nuclear translocation of a number of signal molecules could be indeced rapidly when LPS stimulates the VECs directly.The phosphorylation of CREB and the different activation time coruse among the members of NF-kB are reported for the first time.
出处
《西北国防医学杂志》
CAS
2001年第1期2-5,共4页
Medical Journal of National Defending Forces in Northwest China
基金
国家自然科学基金重点资助项目! (3 983 0 40 0 )
