摘要
目的：探讨内毒素、烫伤血清对培养的心脏微血管内皮细胞（ＣＭＥＣ）间连接成分ＶＥ－钙粘附分子表达的影响，研究内毒素对严重烧伤后内皮细胞的损伤机制。方法：①ＣＭＥＣ的分离培养；②烫伤血清和内毒素刺激培养的ＣＭＥＣ后ＥＬＩＳＡ测定内皮细胞间连接成分ＶＥ－钙粘附分子的表达。结果：ＣＭＥＣ经２０％的烫伤血清刺激后，６ｈ开始ＶＥ－钙粘附分子的表达即有明显减少，是正常对照组的６５％，到２４ｈ仍在低的水平（Ｐ＜０．０１）；经内毒素（２ｍｇ／Ｌ）刺激，从１ｈ开始即有显著降低，是对照组的８０％，６ｈ降低到最低点，是对照组的５６％，后仍在低的水平（Ｐ＜０．０１）。不同浓度的内毒素（０．５、１．０、１．５和２．０ｍｇ／Ｌ）对培养的ＣＭＥＣ进行刺激，作用１２ｈ后，与正常对照组相比差异显著（Ｐ＜０．０５和Ｐ＜０．０１），分别为对照组的８８％、８３％、８０％和４７％，且２．０ｍｇ／Ｌ刺激组有更明显的降低；相同浓度的内毒素（均为２．０ｍｇ／Ｌ）刺激，有血清和不含血清组差异不明显（Ｐ＞０．０５）。结论：烫伤血清和内毒素可以影响内皮细胞中ＶＥ－钙粘附分子的表达，内毒素的影响有剂量的依赖关系，有无血清的存在对ＶＥ－钙粘附分子表达的影响差异不明显?
Objective: To explore the effects of endotoxin and burn serum on the expression of endothelial VEcadherin, an intercellular junctional component, of the isolated and cultured cardiac microvascular endothelial cells. Methods: ①The cardiac microvascular endothelial cells were isolated and cultured. ② ELISA was employed to determine the expression of endothelial VEcadherin, after the cultured cardiac microvascular endothelial cells were stimulated with burn serum and endotoxin. Results: After the stimulation by 20% burn serum, the expression was obviously decreased from the 6th h (P<0.01) and remained low at the 24th h postburn (P<0.01). After the stimulation of 2 mg/L endotoxin, the expression was markedly decreased from the 1st h, reached its valley at 6th h and remained low thereafter postburn (P<0.01). The cultured cardiac microvascular endothelial cells were stimulated by different concentrations of endotoxin for 12 hours. The results showed that the expressions with endotoxin at concentrations of 0.5,1.0,1.5 and 2.0 mg/L were different from that in the control (P<0.05 and P<0.01), i.e.88%,83%,80% and 47% of that in normal respectively. Under the action of the same concentration of endotoxin, no marked difference was found between the expression in the sample with and without serum (P>0.05). Conclusion: The burn serum and endotoxin exert effects on the expression of VEcadherin of the endothelial cells and endotoxin does this in a dosedependent manner. The serum stimulation is not an important factor for the expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第2期108-110,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
内毒素
心脏微血管
内皮细胞
细胞间连接
烫伤
endotoxin
burn
cardiac microvascular endothelial cell
intercellular junction
VEcadherin
rat
