摘要
目的 证实甲硝唑可经口腔吸收进入体内 ,了解其在体内的吸收和排泄情况 ,为含甲硝唑口腔卫生用品的安全性评价提供依据。方法 采用 SD大鼠 ,经结扎食管 30 min后口腔给予含 8%甲硝唑的牙膏 1mg/g(体重 ) ,于给药后0 .5、1、1.5、2、2 .5、3、4.5 h取血 ,应用高效液相色谱法测定血中甲硝唑含量 ,并进行药代动力学分析。结果 口腔给药后 ,0 .5 h血清中即可测得甲硝唑 6 .88μg/ml,说明甲硝唑可经口腔粘膜吸收。血清药 -时曲线拟合表明符合一级吸收一室模型 :C=10 .1419(e- 0 .43 3 7t- e- 1 0 .2 571 t) ,处理后的各项药代动力学参数为 :A=10 .144 9μg/ml;Ke=0 .4337h;Ka=10 .2 5 71h;L ag time=0 .3770 h;t1 / 2 (ka) =0 .0 6 76 h;t1 / 2 (ke) =1.5 984h;T(peak) =0 .32 2 0 h;C(max) =8.44 96 μg/ml;AUC=2 2 .40 43μg/(ml·h) ;CL/F(S) =1.42 93mg/(kg· h) ;V/F(C) =3.2 936 (mg/kg)。结论 甲硝唑可经口腔粘膜吸收进入血液。鉴于甲硝唑的遗传毒性作用 ,建议禁止或限制将甲硝唑加入口腔卫生用品中 ,以免人们长期接触产生不良后果。
Objective To verify the absorption of metronidazole through mouth cavity in vivo,and understand the absorption and excretion of meltronidazole in vivo,and provide scientific bases for the safety assessment on oral health care products including metronidazole Methods The determination of contents of metronidazole in blood by HPLC and pharmacokinetics analysis were carried out in SD rats at the 0 5 h,1 h,1,5 h,2 h,2 5 h,3 h and 4 5 h after the oral exposure to the toothpaste containing 8% metronidazole after the 30 minute ligation of esophagus with a dose of 1 mg/kg body weight (BW) respectively Results 6 88 μg/ml metronidazole was found in serum of SD rats at the 0 5 h after the oral exposure,which indicated that metronidazole in toothpaste could be absorbed through mucous membrane The fited drug concentration in serum time curve accorded with the one compartment model of first grade absorption [C=10 1419(e-0 4337t-e-10 2571t)] The treated parameters of pharmacokinetics were as follows,A=10 1449 μg/ml,Ke=0 4337 h,Ka=10 2571 h;Lag time=0 3770 h,t1/2(ka)=0 0676 h,t1/2(ke)=1 5984 h,T(peak)=0 3220 h,C(max)=8 4496 μg/ml,AUC=22 4043 μg/(ml·h),CL/F(S)=1 429 3 mg/(kg·h),V/F(C)=3 2936 mg/kg Conclusion Metronidazole could be absorbed through oral exposure Due to the genotoxicity of metronidazole,it was proposed that the application of metronidazole in oral health care products should be prohibited and limited,to avoid causing adverse health effects of long term exposure to it
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2001年第2期80-82,共3页
Journal of Environment and Health
基金
天津市卫生局科技基金资助项目 (99KY0 1 9)
关键词
牙膏
甲硝唑
代谢动力学
安全性评价
口腔卫生用品
Toothpaste
Metronidazole
Metabolic dynamics
Safty assessment
Oral health care products
