摘要
从蓝藻Ｃａｌｏｔｈｒｉｘｓｐ．ＰＣＣ７６０１染色体ＤＮＡ中分离出含藻蓝蛋白基因ｃｐｃＢ２Ａ２的略３．８ＫｂＤＮＡ片段，与质粒ｐＵＣ１８重组，构建成一种重组质粒ｐＰＣ２１８－ＰＣＺ，转化Ｅ．ｃｏｌｉＪＭ１０９，获得了一系列克隆子．经斑点分子杂交和限制性内切核酸酶分析，筛选出初步认为含有ｃｐｃＢ２Ａ２基因的克隆子．
Biddulphia regia was collected from Xiamen coast in August 1987.Sexual process of this diatom was observed. Sex Differentiation occured only once a day that lasted 4 hours with the light induction. When the cell size reduced to the range of 26～62 μm in widths ,the sexual process set in ,There were 128 sperms in each spermatogonangum. The survival rate of sperms declined greatly 19 hours after they were formed. Each oogonium contained two eggs. Sperms and eggs were produced at the same time. The results indicated that propaly in this diatom sexualization took place separately in the cells of small size range and middle size range, and it involved two times of restoration of cell sze. The similarities and differences in sexual reproduction showed the relationship of species.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第4期608-612,共5页
Journal of Xiamen University：Natural Science
