摘要
目的：探讨神经生长因子（ NGF）对兔骨折愈合过程中骨形态发生蛋白（ BMP）表达的影响。方法健康新西兰大耳白兔108只。随机分为A组（ NGF组）、B组（拮抗NGF组）、C组（对照组），每组36只。制备标准的兔右侧桡骨骨折模型。 A组采用肌肉注射NGF；B组采用腹腔注射NGF抗体；C组注射生理盐水。各组动物分别于术后24、48 h和1、3、6、8周时处死，测定浓度后进行BMP蛋白免疫印迹分析和图像分析，方差分析及析因设计方差分析检验。结果骨组织中BMP与内参β肌动蛋白灰度值的比值在各个时相点，NGF组比抗NGF抗体组、对照组诱导骨组织内BMP表达量多，且NGF组骨组织中BMP表达时间延长，高峰浓度提前、下降缓慢[NGF组（1.73±0.05），抗NGF组（0.72±0.06），对照组（1.19±0.05），P＜0．05]。结论神经生长因子促进骨折愈合的机制可能是通过促进上调BMP的表达促进骨折愈合。
Objective To explore the regulation of the expression of bone morphogenetic protein （BMP） of nerve growth factor （ NGF） and examine the mechanism of nerve growth factor in promoting fracture repair.Methods A total of 108 healthy New Zealand white rabbits , weighting 2.5-3.0 kg, male or female, were randomly divided into 3 groups of NGF （Group A）, antagonistic NGF（Group B）and control （Group C）（n=36 each）.A standard model of right mid-piece radial fracture was established.Group A received an intravenous injection of NGF 200 ng/kg twice daily 1 week while group B an intraperitoneal injection of NGF antibody 400 ng thrice daily for 3 weeks;group C physiological saline 1 ml twice daily for 3 weeks.And 6 rabbits in each group were sacrificed at 24 h, 48 h, 1 week, 3 weeks, 6 weeks and 8 weeks respectively.And BMP proteins of fracture specimens were analyzed by Western immunoblot.Results NGF promoted the expression of BMP in fracture while NGF antagonist inhibited its expression.NGF group produced more BMP earlier than control or NGF antagonist group during fracture healing while the production of BMP was more pronounced in control group than that in NGF antagonist group during fracture healing .And the differences were statistically significant （ P 〈0.05 ）.Conclusion Capable of promoting the expression of BMP in radial fracture of rabbit , NGF may be beneficial to fracture healing directly or indirectly.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第23期1825-1828,共4页
National Medical Journal of China
关键词
神经生长因子
抗体
形态发生
骨折愈合
Nerve growth factor
Antibodies
Morphogenesis
Fracture healing
