摘要
目的研究血管内皮生长因子(VEGF)及其受体Flt1在下颌骨骨折愈合中的表达规律。方法采用新西兰大耳白兔25只,随机分为术后48h和1、3、5、8周5个时相组,每组5只,以健侧为对照,制作左侧下颌骨体部骨折模型,利用原位杂交、免疫组织化学方法检测VEGF及其受体Flt1在骨折端的表达情况。结果在伤后48h^8周VEGF mRNA及VEGF在骨折部位被多种细胞表达,其表达强度随时间变化而变化,以1~3周为表达的高峰期。在伤后48h^8周的骨折端不同细胞内检测到VEGF的受体Flt1,1~3周为表达高峰期。结论VEGF及其Flt1受体表达贯穿于骨折愈合的始终,VEGF可能在骨折愈合过程中起重要作用。
Objective：To study the expression of vascular endothelial growth factor（VEGF） and its receptor Fltl in the process of mandibular fracture healing. Methods：The fracture models were made in the middle of the left mandible of 25 rabbits,then the specimens from the fracture site were taken 48 hours and 1,3,5 and 8 weeks after operation respectively. The expression of VEGF and Fltl was detected by immunohistochemistry and in situ hybridization. Results： VEGF mRNA, VEGF and Fltl were detected in many kinds of cells in the fracture site from 48 hours to 8 weeks after fracture, and high expression of VEGF and Fltl were detected from 1 to 3 weeks after facture. Conclusions：VEGF and Fltl are expressed through the process of fracture healing, VEGF may play important role in the process of blood vessel rebuilding after fracture.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期179-182,共4页
Journal of Practical Stomatology
基金
吉林省自然科学基金资助项目(20030539-2)
关键词
骨折愈合
血管内皮生长因子
原位杂交
Fracture healing
Vascular endothelial growth factor
Situ hybridization
