摘要
[目的]比较骨形态发生蛋白2(BMP-2)基因治疗与生长因子缓释方法修复节段性骨缺损效果。[方法]于兔双侧桡骨中段造成1·5cm骨缺损,采用4种方法修复:A组植入转基因骨髓间质干细胞(MSCs)与PLA/PCL(聚乳酸/聚己内酯)支架的复合物;B组植入单纯MSCs与含重组BMP-2的PLA/PCL缓释载体的复合物;C组植入单纯MSCs与PLA/PCL复合物;D组植入单纯PLA/PCL。术后4、8、12周行X线、组织学、生物力学和骨密度等检测。[结果]A组体内植入4周后,成骨细胞和间质细胞呈BMP-2强阳性表达;其成骨速度及成骨质量均明显优于B组,12周时骨缺损完全修复。C组成骨能力较弱,而D组则无新骨形成,残留骨缺损。[结论]BMP-2基因治疗是修复节段性骨缺损的好方法。
[ Objective] To compare the effects between gene therapy and gradual release carrier for bone morphogenetic protein-2 （BMP-2） in repairing bone defects, [ Method ] Bone defects of 15 mm were created on the bilateral radius in rabbits and treated with four kinds of implantations, ie, composition of transgeneie MSCs and PLA/PCL （ Group A）, composition of MSCs and gradual release carrier for BMP-2 （Group B）, composition of MSCs and PLA/PCL （Group C）, and PLA/PCL alone （Group D）. After4, 8, and 12 weeks from the operations, X-ray, histological examination, biomechanics analysis, and bone density measurement were conducted. [ Result] After 4 weeks from the operations, in Group A both osteoblasts and mesenchymal cells displayed strongly positive expression of BMP-2 ; the speed and quality of bone formation in Group A were much better than those in Group B, After 12 weeks form the operations, bone defects were completely repaired in Group A. [ Conclusion] BMP-2 gene therapy is really a good method to repair segmental bone defects.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2005年第17期1334-1336,共3页
Orthopedic Journal of China
基金
国家自然科学基金课题(No.39800151)
吉林省科技发展计划(NO.20010110)
