摘要
[目的]建立快速、有效检测农产品中玉米赤霉醇（ZER）残留量的方法。[方法]试验以人工合成的ZER为包被原，ZER为竞争半抗原，两者与一定量的抗玉米赤霉醇单克隆抗体反应，应用间接竞争ELISA法建立玉米赤霉醇单克隆抗体快速检测农产品中ZER含量的半定量检测，同时对该ELISA试剂盒进行了调试。[结果]试验建立检测ZER的ELISA方法，其最适包被浓度为1：5000，抗体最适工作浓度为1：2500，酶标二抗的最适工作浓度为1：5000。试剂盒检测限为0．5ng／ml，添加回收率在70．0％-116．0％，变异系数小于20％，在2～8℃条件下可保存90d。[结论]该试验建立的方法可应用于玉米、小麦、高粱等中的玉米赤霉醇的检测，应用前景广阔。
[ Objective ] To establish a method for rapidly and effectively detecting ZER in agricultural products. [ Method ] With ZER package as original, ZER as hapten for competition, both with a certain amount of resistance to zeranol monoclonal antibody response. The indirect competitive ELISA method was used to establish the zeranol monoclonal antibody rapid detection ZER content in the semi-quantitative detection of agricultural products. At the same time the ELISA kit was tested. [ Result] ELASA method was established to detect ZER, the optimal con- centration of package, IgG-HRP are 1 ： 5 000, the optimal concentration of antibody is 1 ： 2 500. The detection limit of kit is 0.5 ng/ml, adding recovery rate 70.0% - 116.0%, variation coefficient is less than 20%, which can be stored for 3 months under 2 - 8 ℃. [ Conclusion] The method can be used in detection of ZER in maize, wheat, sorghum with broad prospect.
出处
《安徽农业科学》
CAS
2014年第20期6764-6766,6837,共4页
Journal of Anhui Agricultural Sciences
