摘要
目的合成玉米赤霉烯酮(ZEN)的全抗原。方法将烯醇化的ZEN与阳离子化的载体蛋白(BSA)偶联,制备高特异性的全抗原,用高性能基质辅助激光解吸电离-飞行时间质谱仪MALDI-TOF检测偶联物及载体蛋白的分子量,用商品化的ZEN免疫试剂盒对偶联物进行棋盘滴定验证。结果全抗原及载体蛋白BSA的分子量分别为69693.5 Da、66297.7 Da,平均每个BSA连上的ZEN分子个数为10.68个;平均每个OVA上连上的ZEN分子个数为5.32个;偶联物与免疫试剂盒中ZEN的抗体呈阳性反应。结论合成的ZEN全抗原为ZEN抗体的制备及免疫学方法的建立奠定了基础。
Objective To synthesize the complete antigen of zearalenone (ZEN). Method ZEN was coupled with cationic bovine serum albumin (cBSA) via a Mannich reaction. Molecular weights of the complete antigen and carrier protein were measured by MALDI-TOF, respectively. And the performance of complete antigen was further validated by chequerboard titration using a commercial ZEN immunoassay kit. Results The molecular weights of the conjugate and BSA were 69693.5 Da, 66297.7 Da, respectively. There were 10.68 ZEN mole-cules attached to each BSA molecule, and 5.32 ZEN molecules attached to each OVA molecule. A positive re-sult of the immunoreaction between the synthesized complete antigen and the anti-ZEN antibody from immu-noassay kit was observed. Conclusion The preparation of complete antigen of ZEN contributes to the anti-ZEN antibody production and immunoassay development.
出处
《食品安全质量检测学报》
CAS
2014年第3期791-795,共5页
Journal of Food Safety and Quality
基金
国家自然科学基金项目(81102133)~~
