摘要
考察了10 L罐规模条件下pH、温度、装液量以及碳源流加模式对毕赤酵母高密度培养表达鸡α-干扰素的影响,确定了最优的生产工艺:在pH5.5、28℃、40%装液量条件下,当初始碳源耗尽的情况下,进行甘油和甲醇混合(流加体积速率比为20∶1)脉冲流加,产物鸡α-干扰素抗病毒活性最高,可达3×106 IU/mL。将该工艺在50 L发酵罐规模条件下进行放大,重复20批,结果表明此过程重复性较好,且目的蛋白表达量稳定,具有工业化生产的实际应用价值。
To achieve excellent performance of Pichia pastoris for chicken alpha interferon expression, pH value, temperature, culture volume and carbon source feeding pattern were optimized. The maximum anti-virus activity（3×106 IU/mL）was harvested in the conditions of pH5.5, 28℃, and 40%culture volume in 10 L fermentor, as well as the glycerol-methanol（20∶1, V/V）co-feeding pattern was conducted when carbon source exhausted. This process was successfully scaled up to 50 L fermentor and the fermentation performance was also steady, which could be taken advantage of for its industrial application.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第6期115-119,共5页
Biotechnology Bulletin
基金
山东省2011年科技发展计划资助项目(2011GGH22109)
关键词
毕赤酵母
鸡α-干扰素
优化
放大
Pichiapastoris
Chicken alpha interferon
Optimization Up-scale
