摘要
利用NBS公司的BioFloⅢ发酵罐，采用培养基流加的方式培养表达人干扰素的E．coli工程菌，结果湿菌重达128g／L，干扰素活性达98×1010U／L发酵液。
An engineering recominant human interferon producing strain of E. coli was culturedin a 5L BioFlo III fermentor (NBS) by means of now - feeding into the medium during thefermentation. The results were 128g/L of cell weight and 9. 8 ×1010 U/L of interferon ac-tivity respectively.
出处
《微生物学杂志》
CAS
CSCD
1999年第1期24-26,共3页
Journal of Microbiology
关键词
重组
干扰素
大肠杆菌
高密度
高表达
发酵
recombinant human interferon, E. coli, high density, high expression level, fermentation
