摘要
通过单因素优化实验,研究了结冷胶分批发酵过程中初始碳源的选择以及补加碳源的各种工艺条件.结果显示,结冷胶发酵的最佳初始碳源为麦芽糖,最佳初始碳源浓度为6%,补加碳源的最佳方式为在发酵第18h时,一次性补加2.5%的麦芽糖,此时结冷胶产量达13.86g/L,较出发菌株提高了18.8%.
In this paper,selection of initial carbon sources of gellan gum batch fermentation and technological conditions of adding carbon sources were studied.By single factor optimization,the results showed that maltose was the best initial carbon source,and its best concentration was 6%.By the study on technological conditions of adding carbon sources,it was determined that the best way of maltose addition was one-time adding with concentration of 2.5% when the fermentation time was 18 h,the yield of gellan gum was 13.86 g/L,and it＇s increased by 18.8% than the original strain.
出处
《河南大学学报（自然科学版）》
CAS
北大核心
2013年第1期57-61,共5页
Journal of Henan University:Natural Science
基金
2011年河南省科技攻关重点项目(112102210297)
关键词
结冷胶
分批发酵
初始碳源
补加碳源
gellan gum
batch fermentation
initial carbon sources
adding carbon sources
