摘要
目的：研究匙羹藤Gymnema sylvestre（Retz．）Schuh叶中化学成分。方法：应用硅胶、ODS柱色谱和制备HPLC等方法进行分离纯化，应用化学方法结合1H-NMR和13-C-NMR波谱技术鉴定化合物结构，测定化合物的体外蛋白非酶糖基化抑制活性，其中化合物4在50mg·L-1时有较好抑制作用，抑制率为42．11％。结果：从匙羹藤叶中分离得到5个化合物，分别为灰毡毛忍冬皂苷乙（1）、灰毡毛忍冬皂苷甲（2）、lonimacranthoideⅢ（3）、朝霍定C（4）、3-0-β-D-吡喃葡萄糖基-（1→3）-a-L-吡喃鼠李糖基-（1→2）-α-L-吡喃阿拉伯糖基-常春藤皂苷元-28-0-β-D-毗喃葡萄糖基酯（5）。评价了化合物1—5的体外蛋白非酶糖基化抑制活性。结论：化合物1-5均为首次从本属植物中分离得到，体外活性筛选表明化合物4在高浓度时具有一定的蛋白非酶糖基化抑制作用。
Objective： To study the chemical constituents of Gymnema sylvestre. Method： By column chromatopraphy of silica gel, ODS and prep-HPLC, etc, compounds were separated and purified. Structural analysis was carried out by chemical methods, spectroscopic （1H-NMR,13 C-NMR） and MS analysis. Result： Five compounds were isolated from the leaves of G. sylvestre. Their structures were elucidated as macranthoidin B （1）, macranthoidin A （2）, lonimacranthoide Ⅲ （3）, epimedin C （4）, and 3-O-β-D-glncopyranosyl- （1→3） - α-L-rhamnopynosyl- （ 1 → 2 ） -α-L-arabinopyranosyl-hederagenin-28-O-β-D-glucopyranosyl ester （ 5 ）. The inhibition activity of compounds 1-5 on non-enzymatic glycation of protein in vitro were evaluated by calculating the inhibitory rates of them and control groups on the basis of determining the fluorescence intensity of advanced glycation end products （ AGEs）, ie, reaction products of bovine serum albumin （BSA） and glucose after a week at37 ℃. Conclusion： Compounds 1-5 were compounds 4 at a concentration of 50 mg ·L-1 have amedium inhibition activity for non-enzymatic glycation of protein in vitro.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期102-106,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部国际科技合作项目(2010DFB33260)
吉林省科技厅项目(20100122)
关键词
匙羹藤
化学成分
体外蛋白非酶糖基化抑制活性
Gymnema sylvestre
chemical constituents
non-enzymatic glycation of protein inhibitionactivity in vitro
