摘要
目的：建立同时测定淡豆豉中6种异黄酮类成分的分析方法，并研究炮制工艺对淡豆豉药材中异黄酮类成分的影响。方法：自行发酵淡豆豉并采用高效液相色谱法测定其异黄酮含量，使用岛津SB-C_18 HPLC色谱柱（4．6mm×150mm，5μm）；以0．2％甲酸乙腈-0．2％甲酸水溶液为流动相，梯度洗脱；采用全波长紫外检测器扫描；流速1mL·min-1，柱温35℃。结果：淡豆豉中各异黄酮色谱峰的分离度良好，在一定范围内与峰面积积分值呈良好的线性关系（r〉0．999），平均加样回收率在93．16％～109．98％，RSD均〈3．76％，并发现炮制过程中苷含量逐渐降低，苷元含量逐渐升高。结论：该方法操作简便、稳定、可靠，可用于淡豆豉多指标成分的含量测定，同时证明6～8d的发酵条件最利于苷类成分的水解，较适合淡豆豉的发酵。
Objective. To establish an HPLC method for content determination of 6 kinds of isoflavones in Sojae Semen praeparatum and to optimize the processing technology of Sojae Semen praeparatum. Method： Fermentation technique was used to produce Sojae Semen praeparatum; SHIMADZU SB-Cls HPLC Column （4.6 mm × 150 mm, 5 μm） was used with the mobile phase of O. 2% formic acid acetonitrile-0.2% formic acid at a flow rate of 1 mL .rain-1 The detection wavelength was set at 260 nm and the column temperature was maintained at 35 ℃. Result： All the compounds were linear （r 〉 0. 999） in the range of the test concentrations, and the average recovery of the method was between 93.16% and 109.98% , RSD 〈3.76% ; meanwhile, the content of glycosides greatly reduced and the content of aglycons increased significantly after processing. Conclusion： The method established by this paper can be used to determine the six kinds of isoflavones in Sojae Semen Praeparatum simultaneously, which can provide a scientific basis for the quality evaluation of Sojae Semen Praeparatum ; and the optimum time for fermentation of Sojae Semen Praeparatum is 6 - 8 days.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期72-76,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省高校自然科学基础研究项目(08KJB360004)
南京中医药大学青年自然科学基金项目(12XZR24)
关键词
淡豆豉
异黄酮
炮制工艺
Sojae Semen Praeparatum
isoflavones
processing technology
