摘要
目的建立淡豆豉药材高效液相色谱指纹图谱(HPLC-FPS),为其质量控制提供依据。方法应用高效液相色谱法(HPLC),利用反相色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm),流动相:乙腈-3%冰乙酸梯度洗脱,流速:1 ml/min,柱温:30℃,检测波长:261 nm,进样量:10μl。结果不同产地淡豆豉在化学成分方面具有明显相似性,可建立淡豆豉的指纹图谱。结论建立的HPLC指纹图谱可用于淡豆豉的质量评价。
Objective To establish a HPLC fingerprint method for Semen Sojae Preparatumto quality control.Methods HPLC analysis was performed on a Diamonsil C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was used in gradient elution consisted of methanol-water（3% acetic acid）,the flow rate was 1.0 ml /min,column temperature was 30℃ and UV detected was set at 261 nm,the sample weight：10 μl.Results The compound composition of different SSP origins was obviously comparable,which could be determined by HPLC fingerprint of the SSP.Conclusion This method could be utilized as a quality control measurement for SSP.
出处
《药学实践杂志》
CAS
2012年第5期351-352,386,共3页
Journal of Pharmaceutical Practice
基金
上海市卫生局项目(2010Y006A)
关键词
淡豆豉
高效液相色谱法
高效液相色谱指纹图谱
SSP
high performance liquid chromatography（HPLC）
high performance liquid chromatographic fingerprint（HPLC-FPS）
